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We are seeking a highly motivated and organized Scientist II to contribute to the research and development of monoclonal antibodies in our Antibody Technologies team. This individual will be responsible for working in a dynamic team environment and contributing to multiple work streams, including testing new antibodies for use in flow cytometry applications, hybridoma tissue culture, and recombinant antibody expression. The position requires extensive hands-on experience with flow cytometry, expertise in tissue culture, and an understanding of biochemistry and molecular biology. The successful candidate is expected to set up and execute experiments, be detail-oriented, skilled at data analysis and troubleshooting, and accountable for preparing and presenting data at lab meetings with guidance regarding required tasks and expected results.
Job Responsibility:
Execute experiments with varying complexity using multi-color Flow Cytometry
Analyze data using FCS Express and FlowJo software
report test results, including completion of all required documentation and database entries as required by protocols and SOPs
Responsible for transfection and expression of antibodies and recombinant proteins in cell lines, hybridoma tissue culture, and culture of various cell lines for testing
Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met
Independently analyze research data, solve problems with minimal guidance, and effectively plan follow-up experiments
Involvement with scheduling, operational, and maintenance duties required within the laboratory
Requirements:
Minimum of 2-5 years of laboratory experience required
Extensive hands-on experience with flow cytometry
Proficiency in tissue culture techniques, including transfection of cells for protein expression
Understanding of biochemistry and molecular biology
Hands-on experience designing, performing, and analyzing flow cytometry experiments, preferably on BD flow cytometers (LSRII, LSR Fortessa, Canto, Aria)
Proficient in tissue culture techniques
Familiarity with molecular biology (DNA preparation)
Software proficiency: BD FACSDiva, FlowJo, FCS Express, and Microsoft Office Suite
Effective interpersonal skills and ability to work in a cross-functional team-oriented environment
Strong written and oral communication skills
Ability to follow established policies and procedures and maintain neat, accurate, and complete records
Effective analytical and problem-solving skills
A Bachelor’s or Master of Science degree in cellular or molecular biology, immunology, cell biology, or a related scientific discipline
Minimum 2 years of lab experience
Flow cytometry and tissue culture experience required
Nice to have:
Preferred: Familiarity with assay automation and automated liquid handlers