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The Associate Research Specialist (ARS) will be involved in pediatric neural cancer research at Children's Hospital Los Angeles in the Smith Research Tower in Dr. Huang's Laboratory. The lab is focused on understanding the genetic causes of and identifying potential therapeutic targets for two pediatric neural tumors: medulloblastoma and neuroblastoma. This is an ideal position for a self-motivated individual interested in expanding skill sets in a wet laboratory setting and/or pursuing a PhD or MD/PhD in the future.
Job Responsibility:
Conducts routine laboratory research procedures
Follows protocol to perform experiments
Tabulates data and may assist with analysis
Performs routine equipment maintenance and orders supplies
May provide instruction to support personnel
Maintain and differentiate human stem cells
Clone DNA constructs
Perform genome editing using CRISPR
Perform transduction/transfections, flow cytometry, western blot, RT-qPCR, immunofluorescence, immunohistochemistry and mouse surgeries
Rotate with 2-3 other lab mates to maintain cell cultures over the weekends
Present at lab meetings, lead a project(s) and be a co-author or first author on publication(s)
Requirements:
Experience working in a laboratory environment required
Preferred 1+ years experience performing laboratory research (previous work in a biology research lab for at least 6 months and have at least 3 months of experience performing mammalian cell culture)
Previous experience working with live mice and human stem cells is preferred
Bachelor’s degree or equivalent experience in a scientific field
Applicants must have completed their bachelor’s degree by their anticipated start date
Candidates must be committed to working for at least 2 years
Nice to have:
Previous experience working with live mice and human stem cells